Associate Member, Monell Chemical Senses Center
Professor, Zhejiang University, China
PhD, Molecular Biology; Yale University
My research is directed at the molecular mechanisms underlying taste and smell signal transduction and receptor cell regeneration. The focus is on identifying and characterizing molecules that are involved in the recognition and transmission of taste and olfactory stimuli in the oral and nasal cavities, respectively, and on the progenitor cells’ differentiation and maturation in the olfactory epithelium. This work has implications for understanding the cellular and molecular basis of chemosensation under various circumstances, which may lead to effective treatments of such taste and olfactory disorders as dysgeusia and anosmia.
single cells, gene expression, signal transduction, chemosensory perception, transgenesis, anosmia
Du, L.; Zhao, L.; Huang, L.; Wang, P.; Wu, C. (2014) Label-freefunctional assays of chemical receptors using a bioengineered cell-based biosensor with localized extracellular acidification measurement. Biosensors and Bioelectronics, 54, 623-627.
Feng, P.; Chai, J.; Zhou, M.; Simon, N.; Huang, L. ; Wang, H. (2014) Interleukin-10 is produced by a specific subset of taste receptor cells and critical for maintaining structural integrity of mouse taste buds. Journal of Neuroscience, 34, 2689-2701.
Feng, P.; Huang, L.; Wang, H.M. (2014) Taste bud homeostasis in health, disease, and aging. Chemical Senses, 39, 3-16.
Du, L.; Wu, C.; Liu, Q.; Huang, L.; Wang, P. (2013) Recent advances in olfactory receptor-based biosensors. Biosensors and Bioelectronics, 42, 570-580.
Du, L.; Wu, C.; Peng, H.; Zhao, L.; Huang, L.; Wang, P. (2013) Bioengineered olfactory sensory neuron-based biosensor for specific odorant detection. Biosensors and Bioelectronics, 40, 401-406.
Li,F.; Ponissery Saidu,S.; Yee,K.K.; Wang,H.; Chen,M-L.; Iguchi,N.; Zhang,G.; Jiang,P.; Reisert,J.; Huang,L. (2013) Heterotrimeric G Protein Subunit Ggamma13 Is Critical to Olfaction. The Journal of Neuroscience, 33, 7975-7984.
Wu, C.; Du, L.; Zou, L.; Huang, L.; Wang, P. (2013) A biomimetic bitter receptor-based biosensor with high efficiency immobilization and purification using self-assembled aptamers. Analyst, 138, 5989-5994.
Xu, J.; Cao, J.; Iguchi, N.; Riethmacher, D.;Huang, L. (2013) Functional characterization of bitter taste receptors expressed in mammalian testis. Molecular Human Reproduction, 19, 17-28.
Zhu, X.; Li, Z., Jiang, D.; Zhao, J.; Huang, L.; Zhang, J.; Huang, X. (2012) Characterization and expressional analysis of Dleu7 during Xenopus tropicalis embryogenesis. Gene, 509, 77-84.
Feng, P.; Zhao, H.; Chai, J.; Huang, L.; Wang, H. (2012) Expression and secretion of TNF-a in mouse taste buds: a novel function of a specific subset of type II taste cells. PLoS ONE, 7, e43140.
Kim, A.; Feng, P.; Ohkuri, T.; Sauers, D.; Cohn, Z.J.; Chai, J.; Nelson, T.; Bachmanov, A.A.; Huang, L.; Wang, H. (2012) Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease. PLoS ONE, 7, e35588.
Iguchi N.; Ohkuri, T.; Slack, J. P.; Zhong, P. and Huang, L. (2011) Sarco/endoplasmic reticulum ca2+-atpases (SERCA) contribute to gpcr-mediated taste perception. PLoS ONE, 6, e23165.
Cohn, Z. J.; Kim, A.; Huang, L.; Brand, J. and Wang, H. (2010) Lipopolysaccharide-induced inflammation attenuates taste progenitor cell proliferation and shortens the life span of taste bud cells. BMC Neuroscience, 11, 72.
Hunter-Smith, S. and Huang, L. (2009) Human taste: peripheral anatomy, taste transduction and coding. AgroFood Industry Hi-Tech, 20, 24-27.
Maehashi, K. and Huang, L. (2009) Bitter peptides and bitter taste receptors. Cellular and Molecular Life Sciences, 66, 1661-1671.
Rawson, N. E. and Huang, L. (2009) Symposium overview: Impact of oronasal inflammation on taste and smell. Annals of the New York Academy of Sciences, 1170, 581-584.
Wang, H.; Iguchi, N.; Rong, Q.; Zhou, M.; Ogukunorode, M.; Inoue, M.; Pribitkin, E. A.; Bachmanov, A. A.; Margolskee, R. F.; Pfeifer, K. and Huang, L. (2009) Expression of the voltage-gated potassium channel KCNQ1 in mammalian taste bud cells and the effect of its null-mutation on taste preferences. Journal of Comparative Neurology, 512, 384-398.
Wang, H.; Zhou, M.; Brand, J. and Huang, L. (2009) Inflammation and taste disorders: mechanisms in taste buds. Annals of the New York Academy of Sciences, 1170, 596-603.
Huang, L. (2008) Bitter taste for flavor and health. Perfumer and Flavorist, 33, 52-55.
Naim, M; Shaul, M. E.; Spielman, A. I.; Huang, L. and Peri, I. (2008) Permeation of amphipathic sweeteners into taste-bud cells and their interactions with post-receptor signaling components: Possible implications for sweet-taste quality. In: Weearsinghe, D.K.; Dubois, G. (Ed.s) Sweetness and Sweeteners: Biology, Chemistry, and Psychophysics. London: Oxford University Press, 241-255.
Maehashi, K.; Matano, M.; Wang, H.; Vo, L.A.; Yamamoto, Y.; Huang, L. (2008) Bitter peptides activate hTAS2Rs, the human bitter receptors. Biochemical and Biophysical Research Communications, 365, 851-855.
Wang, H.; Zhou, M.; Brand, J.; Huang, L. (2007) Inflammation activates the interferon signaling pathways in taste bud cells. Journal of Neuroscience, 27, 10703-10713.
Breslin, P.A.S.; Huang, L. (2006) Human taste: peripheral anatomy, taste transduction and coding. Advances in Otorhinolaryngology, 63, 152-190.
Huang, L.; Cao, J.; Wang, H.; Vo, L.A.; Brand, J.G. (2005) Identification and functional characterization of a voltage-gated chloride channel and its novel splice variant in taste bud cells. Journal of Biological Chemistry, 280, 36150-36157.