There are five components, four to make the tubes, and one for the cage:
The sipper tube provides the link between the solution and the mouse tongue. One end of the tube must make a watertight fit with a graduated fluid reservoir. The other end must allow taste solution to be obtained by the mouse. It must resist the chemical actions of taste solutions, including acids and salts. It must also be able to withstand chewing, scratching, and gnawing. In practice, the only available material to do this is stainless steel.
The size of the hole through which the mouse licks fluids is critical. Differences in the diameter of this hole can influence licking rates and fluid intakes. If the hole is too large, spillage increases. If it is too small, the mouse has difficulty licking viscous fluids. It is standard for this hole to be 1/8 in diameter.
Some laboratories use sipper tubes with larger holes but a stainless steel ball. These have the advantage of reducing spillage but they are (a) more expensive, (b) difficult to clean, and (c) occasionally block.
Length: At least 1.75 (45 mm)
Tube outer diameter 5/16 (7.94 mm)
Wall thickness .028 (0.71 mm)
Hole diameter (where mouse licks) 0.125 (3.175 mm)
We use sipper tubes manufactured Unifab
The 2.5 (63.5 mm) long stainless steel straight sipper tubes are fine for most needs (Cat. No. US-171-25). However, a shorter tube is a little more efficient, and so we order tubes custom made to be 45 mm (1.75) long.
Depends on the number ordered. In lots of 1000, they cost about $1.25/sipper tube for the 2.5 long tubes; $1.31 for the custom-made 45 mm tubes. Shipping time, about 3-4 weeks (Dec 1999 prices).
Inner diameter, 5/16 (tube with ID ¼ (6.4 mm) will also work but it is a tight fit over the sipper tubes, making assembly a strain).
Outer diameter, ½ (12.8 mm)
Cut into ½ lengths with a tube cutter, scissors or a sharp modeling knife. A plastic tubing cutter (Bel-Art Products, cat no. 21010-0000; Pequannock, NJ 07440-1992) makes the job faster and gives a straighter cut.
Vendor Cole-Parmer, Cat. No. 06411-76 (best) or (less good) 06411-74
25 ft for $75 (enough for about 600 tubes)
Typical water intakes of mice are ~5 ml/day. Intakes of highly preferred solutions such as saccharin can be as high as 25 ml/day. The goal here is to provide at least a days worth of fluid in a graduated container that can be easily read. If the reservoir is too small it must be refilled frequently. If it is too large, it can be either too wide in diameter to allow accurate measurements or too long to be conveniently held on the cage. Extensive attempts to improve on our design has taught us that if the reservoir is too narrow, there is insufficient room for fluid to drain and air to rise to the top, leading to a blockage.
25-ml plastic serological pipettes, with gradations every 0.2 ml.
Fisher cat no. 13-678-14B
Rubber stopper, size 00
Fisher cat no. 14-130A
If the mice can get hold of the silicone seal or plastic reservoir they will chew on them. To prevent this, we install a guard on each cage. This is a thin metal sheet with two holes drilled to accept the drinking spouts. The holes also force the drinking tubes to be placed in the same place each time they are used.
A piece of sheet metal, approx 4 long, with two holes for the drinking spouts and two for nuts and bolts to mount it to the cage lid (see figure)
Alternative: A steel washer around each drinking spout. These are fine for small experiments, but they are not as convenient as the metal strip protector because they do not stay in place
Note the metal strip that stops the mouse from eating the silicone seal
Supplies for Sipper tubes
Contact for custom orders: Bob Boyington
Phone: (800)-648-9569 or (616)-382-2803
Supplies for tubing used for seal between sipper tube and reservoir
Figure 1. A drinking tube assembled (left)
and its components (right)